control non cre dependent aav9 cmv egfp vector Search Results


egfp aav9 viral vector  (Addgene inc)
96
Addgene inc egfp aav9 viral vector
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Egfp Aav9 Viral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/pAAV-hSyn-EGFP+(Plasmid+%2350465)/bio_rxiv__2021__03__03__433128-302-7-16
Average 96 stars, based on 1 article reviews
egfp aav9 viral vector - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
aav9 cmv m rpl3  (Vector Biolabs)
95
Vector Biolabs aav9 cmv m rpl3
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Aav9 Cmv M Rpl3, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/AAV-m-RPL3/pmc12240844-89-6-7
Average 95 stars, based on 1 article reviews
aav9 cmv m rpl3 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
control virus aav9 tmck egfp  (Vector Biolabs)
95
Vector Biolabs control virus aav9 tmck egfp
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Control Virus Aav9 Tmck Egfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/AAV-tMCK-EGFP-WPRE/bio_rxiv__2025__07__30__667744-243-1-9
Average 95 stars, based on 1 article reviews
control virus aav9 tmck egfp - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
scrambled shrna  (Vector Biolabs)
95
Vector Biolabs scrambled shrna
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Scrambled Shrna, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/AAV9-GFP/pmc08011979-199-19-32
Average 95 stars, based on 1 article reviews
scrambled shrna - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
vector delivered cre recombinase  (Virovek Inc)
86
Virovek Inc vector delivered cre recombinase
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Vector Delivered Cre Recombinase, supplied by Virovek Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/cre+delivered+recombinase+vector/pmc11223979-374-3-13
Average 86 stars, based on 1 article reviews
vector delivered cre recombinase - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
recombinant dna reagent aav9 camkiiα eyfp wpre hgh unc vector core 3 9  (Addgene inc)
92
Addgene inc recombinant dna reagent aav9 camkiiα eyfp wpre hgh unc vector core 3 9
a, Live neuronal labeling by expression of hSYN <t>::EGFP</t> delivered by an <t>AAV9</t> vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.
Recombinant Dna Reagent Aav9 Camkiiα Eyfp Wpre Hgh Unc Vector Core 3 9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/pNN6+(Plasmid+%2331012)/10__7554_slash_elife__69293-256-104-126
Average 92 stars, based on 1 article reviews
recombinant dna reagent aav9 camkiiα eyfp wpre hgh unc vector core 3 9 - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
target chr2 aav9 ef1a dio hchr2 h134r eyfp wpre hgh  (Addgene inc)
96
Addgene inc target chr2 aav9 ef1a dio hchr2 h134r eyfp wpre hgh
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Target Chr2 Aav9 Ef1a Dio Hchr2 H134r Eyfp Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/pmc06345483-28-12-15
Average 96 stars, based on 1 article reviews
target chr2 aav9 ef1a dio hchr2 h134r eyfp wpre hgh - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
aav9 syn  (Addgene inc)
95
Addgene inc aav9 syn
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Aav9 Syn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/pGP-AAV-syn-FLEX-jGCaMP7f-WPRE+(Plasmid+%23104492)/pmc11976289-425-20-29
Average 95 stars, based on 1 article reviews
aav9 syn - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
adv-nc  (Shanghai GenePharma)
90
Shanghai GenePharma adv-nc
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Adv Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/ad+nc/pmc11850654-123-7-16
Average 90 stars, based on 1 article reviews
adv-nc - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
aav9 gfap 0 7 m ifnγ ires egfp aav 261933  (Vector Biolabs)
94
Vector Biolabs aav9 gfap 0 7 m ifnγ ires egfp aav 261933
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Aav9 Gfap 0 7 M Ifnγ Ires Egfp Aav 261933, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/AAV-m-IFNG/pm40419671-162-0-9
Average 94 stars, based on 1 article reviews
aav9 gfap 0 7 m ifnγ ires egfp aav 261933 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
aav9 vectors  (Genechem)
86
Genechem aav9 vectors
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Aav9 Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/aav9+vectors/pm40908792-131-49-51
Average 86 stars, based on 1 article reviews
aav9 vectors - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
aav9 cardiac troponin t promoter vector  (Addgene inc)
93
Addgene inc aav9 cardiac troponin t promoter vector
(A) targeting <t>ChR2</t> to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).
Aav9 Cardiac Troponin T Promoter Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+non+cre+dependent+aav9+cmv+egfp+vector/pAAV%3AcTNT%3A%3ALuciferase+(Plasmid+%2369915)/pmc09023524-323-9-14
Average 93 stars, based on 1 article reviews
aav9 cardiac troponin t promoter vector - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


a, Live neuronal labeling by expression of hSYN ::EGFP delivered by an AAV9 vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: A Cultured Sensorimotor Organoid Model Forms Human Neuromuscular Junctions

doi: 10.1101/2021.03.03.433128

Figure Lengend Snippet: a, Live neuronal labeling by expression of hSYN ::EGFP delivered by an AAV9 vector. Scale bar, 100 µm. b, Quantification of neurite area as fold change of initial, from three to nine weeks in culture. Line indicates mean and shaded area indicates S.E.M.(effect of time on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=8.743, P=1.3×10 -10 , individual comparisons: TDP-43 pair: F=37.040, P=2.0×10 -8 ; SOD1 pair: F=16.3725, P=1.0×10 -4 ; PFN1 pair: F=18.639, P=3.6×10 -5 ; interaction between time and iPSC line on neurite area, n =6 organoid cultures obtained from 3 separate differentiations, linear model, F=0.562, P=0.985). c, Normalized percentage of NMJ innervation at three months in culture (normalized to the paired isogenic controls). Bars indicates mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=5.938, P=6.3×10 -4 ; separate two-tailed t-test; TDP-43 pair: P=0.560; SOD1 pair: P=0.010; PFN1 pair: P=0.047). d, Normalized area of the innervated NMJs at three months in culture (normalized to the paired isogenic controls). Bars indicate mean and S.E.M. ( n =6 organoid cultures obtained from 3 separate differentiations identified by colors, Univariate ANOVA: F=3.001, P=0.026; separate two-tailed t-test; TDP-43 pair: P=0.009; SOD1 pair: P=0.203; PFN1: P=0.659). e, Example of the reduction in NMJ area observed in the TDP-43 +/G298S iPSC line compared to the isogenic control. Scale bar, 100 µm.

Article Snippet: Organoid cultures were transduced with an hSYN ::EGFP AAV9 viral vector (a gift from Bryan Roth (Addgene viral prep # 50465-AAV9; http://n2t.net/addgene:50465 ; RRID:Addgene_50465) at week 2 of culture.

Techniques: Labeling, Expressing, Plasmid Preparation, Two Tailed Test, Control

(A) targeting ChR2 to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).

Journal: PLoS ONE

Article Title: Enhanced activity of pyramidal neurons in the infralimbic cortex drives anxiety behavior

doi: 10.1371/journal.pone.0210949

Figure Lengend Snippet: (A) targeting ChR2 to pyramidal cortical neurons and experimental workflow. IL infralimbic cortex (B) group data for openfield experiments in ChR2 injected animals (EXP), center duration Off1 39.49±6.9s, On1 19.87±4.47s, Off2 28.13±8.55s, On2 23.42±9.32s, Off1:On1 two tailed t-test, t = 2.286, p = 0.033, Off1:Off2 Mann Whitney Rank Sum test p = 0.168, Off1:On2 Mann Whitney Rank Sum test p = 0.049, distance moved Off1 2703.09±292.65cm, On1 3113.4±491.15cm, Off2 3331.86±482.62cm, On2 3082.17±658,61cm, Off1:On1 Mann Whitney Rank Sum test p = 0.743, Off1:Off2 two tailed t-test t = 0.52, p = 0.609, Off1:On2 Mann Whitney Rank Sum p = 0.646, n = 11 mice. (C) example of raw data from an individual mouse during an openfield test. (D) group data for novelty suppressed feeding, time till feeding CT no light 112.42±14.9s, CT light 129.38±22.96s, EXP no light 93.38±17.41s, EXP light 225.86±32.76s, CT light:EXP light, two tailed t-test, t = -2.461, p = 0.029, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.004, food intake CT no light 0.12±0.02g, CT light 0.15±0.02g, EXP no light 0.16±0.03, EXP light 0.15±0.02 CT light:EXP light Mann Whitney Rank Sum test, p = 1, EXP no light:EXP light Mann Whitney Rank Sum test p = 0.955. (E) example of raw data from an individual mouse during novelty suppressed feeding test. (F) total amount of c-fos expressing cells, naïve EXP 47.11±3.25, CT 83.86±5, Anx EXP 160.14±10.14, CT 231.21±9.93, Anx+L EXP 323.38±13.61, CT 192.11±9.51. Anx EXP:Anx+L EXP two tailed-t-test t = -9.709, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -8, p≤0.001. Anx anxiety paradigm, Anx+L anxiety paradigm with light stimulation (G) colocalization of c-fos expression in GABAergic neurons (Gad67) naïve EXP 9.93±0.76, CT 16.57±1.17, Anx EXP 21.5±1.2, CT 29.14±1.23, Anx+L EXP 34.12±1.84, CT 27.71±1.49. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT two tailed-t-test t = -2.714, p = 0.009. (H) colocalization of c-fos expression in pyramidal neurons (CamKII) naïve EXP 16.04±1.14, CT 28.21±2.1, Anx EXP 56.68±3.52, CT 71.57±2.98, Anx+L EXP 120.08±542, CT 65.46±3.26. Anx EXP:Anx+L EXP Mann Whitney Rank Sum test, p≤0.001, Anx+L EXP:Anx+L CT Mann Whitney Rank Sum test p≤0.001. (I) activity ratio between pyramidal neurons and interneurons (AR PN/IN), calculated as ratio between c-fos positive pyramidal and c-fos positive GABAergic neurons. Naïve EXP 1.63±0.05, CT 1.7±0.04, Anx EXP 2.65±0.08, CT 2.47±0.05, Anx+L EXP 3.59±0.1 CT 2.38±0.04. Anx EXP: Anx+L EXP two tailed t-test t = -7.377, p≤0.001, Anx+L EXP: Anx+L CT Mann Whitney Rank Sum test p≤0.001.Values are mean ± S.E.M. * indicate significant differences (p≤ 0.05), ** indicate significant differences (p≤ 0.01), *** indicate significant differences (p≤ 0.001).

Article Snippet: Nex-Cre(-/-) mice were injected with AAVs, encoding for Cre depended expression to target ChR2 (AAV9.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (Adgene 20298, Penn Vector Core)) or td-tomato (pAAV1.CAG.FLEX.tdTomato.WPRE.bGH (Penn Vector Core, Adgene 51503)) to pyramidal neurons in the IL region.

Techniques: Injection, Two Tailed Test, MANN-WHITNEY, Expressing, Activity Assay